Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-23
... termini of DNA molecules carry homopolymeric tails of very different lengths . This prob- lem can be alleviated ... cohesive termini compatible with those of the linker . Double - stranded cDNA molecules containing the synthetic cohesive ...
... termini of DNA molecules carry homopolymeric tails of very different lengths . This prob- lem can be alleviated ... cohesive termini compatible with those of the linker . Double - stranded cDNA molecules containing the synthetic cohesive ...
Page 8-24
... cohesive termini will of course ligate to each other as well as to the vector DNA . It is therefore important to ... termini of the cDNA and cleaved with the appropriate restriction enzyme to generate molecules with cohesive termini ...
... cohesive termini will of course ligate to each other as well as to the vector DNA . It is therefore important to ... termini of the cDNA and cleaved with the appropriate restriction enzyme to generate molecules with cohesive termini ...
Page 15-85
... cohesive termini Ligation of a single - stranded hexameric linker to the cohesive termini • Removal of excess linkers by cleavage with the appropriate restriction enzyme Ligation of a fragment of DNA carrying a selectable marker ...
... cohesive termini Ligation of a single - stranded hexameric linker to the cohesive termini • Removal of excess linkers by cleavage with the appropriate restriction enzyme Ligation of a fragment of DNA carrying a selectable marker ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml