Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-57
... coli E. coli DNA ligase E. coli DNA polymerase I EcoRI M.EcoRI methylase EDTA ( 0.5 м ; pH 8.0 ) IPTG For a cuvette with a path length of 1 cm , absorbance = € M . 100 mm stock solutions of each dNTP are commercially available ( Phar ...
... coli E. coli DNA ligase E. coli DNA polymerase I EcoRI M.EcoRI methylase EDTA ( 0.5 м ; pH 8.0 ) IPTG For a cuvette with a path length of 1 cm , absorbance = € M . 100 mm stock solutions of each dNTP are commercially available ( Phar ...
Page 11-40
... coli DNA POLYMERASE I In some cases ( e.g. , when using oligonucleotides as probes in Southern hybridizations ) , it is important to radiolabel to the highest possible specific activity . At best , phosphorylation results in the ...
... coli DNA POLYMERASE I In some cases ( e.g. , when using oligonucleotides as probes in Southern hybridizations ) , it is important to radiolabel to the highest possible specific activity . At best , phosphorylation results in the ...
Page 12-26
A Laboratory Manual Joseph Sambrook, Tom Maniatis. PREPARATION OF E. coli LYSATES FOR ABSORPTION OF ANTI - E . coli ANTIBODIES 1. Grow 100 - ml cultures of E. coli strain Y1090hsdR to saturation in LB medium . 2. Harvest the cells by ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. PREPARATION OF E. coli LYSATES FOR ABSORPTION OF ANTI - E . coli ANTIBODIES 1. Grow 100 - ml cultures of E. coli strain Y1090hsdR to saturation in LB medium . 2. Harvest the cells by ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml