Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-18
... complementary strands of a given sequence of nucleic acid , have a considerable advantage over the more traditional double - stranded probes : The absence of a com- plementary strand eliminates the possibility of forming nonproductive ...
... complementary strands of a given sequence of nucleic acid , have a considerable advantage over the more traditional double - stranded probes : The absence of a com- plementary strand eliminates the possibility of forming nonproductive ...
Page 11-41
... complementary sequences at their 3 ' termini ( Ullrich et al . 1984a ) ( see Figure 11.2 ) . Both strands are extended , resulting in a duplex that may be considerably longer than either of the two original oligonucleotides ( see pages ...
... complementary sequences at their 3 ' termini ( Ullrich et al . 1984a ) ( see Figure 11.2 ) . Both strands are extended , resulting in a duplex that may be considerably longer than either of the two original oligonucleotides ( see pages ...
Page 15-97
... complementary to the opposite strand and that carries the desired mutations . These sets of complementary oligonucleotides are then mixed under conditions that will allow mismatched hybrids to form . If the complementary ...
... complementary to the opposite strand and that carries the desired mutations . These sets of complementary oligonucleotides are then mixed under conditions that will allow mismatched hybrids to form . If the complementary ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml