Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-49
... concentration of 500 μg / ml in hybridization solutions containing dextran sulfate ; in hybridization solutions that do not contain dextran sulfate , heparin is used at a concentration of 50 μg / ml ( Singh and Jones , 1984 ) ...
... concentration of 500 μg / ml in hybridization solutions containing dextran sulfate ; in hybridization solutions that do not contain dextran sulfate , heparin is used at a concentration of 50 μg / ml ( Singh and Jones , 1984 ) ...
Page 10-20
... concentration in excess of K and one radiolabeled dNTP at a lower concentration , which is usually below Km . Under these conditions , the proportion of radiolabel that is incorporated into DNA is very high , even though the rate of the ...
... concentration in excess of K and one radiolabeled dNTP at a lower concentration , which is usually below Km . Under these conditions , the proportion of radiolabel that is incorporated into DNA is very high , even though the rate of the ...
Page 10-32
... concentration . More than 90 % of the transcripts are full - length when the concentration of each of the rNTPs is 50 μм or greater . Because bacteriophage SP6 DNA - dependent RNA polymerase is more tolerant of lower concentrations of ...
... concentration . More than 90 % of the transcripts are full - length when the concentration of each of the rNTPs is 50 μм or greater . Because bacteriophage SP6 DNA - dependent RNA polymerase is more tolerant of lower concentrations of ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml