Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxiv
... Deletions and Insertions 15.3 SIMPLE DELETIONS OR INSERTIONS 15.3 SYSTEMATIC DELETIONS AND INSERTIONS 15.5 Linker - insertion Mutagenesis 15.5 GENERATION OF LINKER - INSERTION MUTANTS WITH FREQUENTLY CUTTING RESTRICTION ENZYMES 15.8 ...
... Deletions and Insertions 15.3 SIMPLE DELETIONS OR INSERTIONS 15.3 SYSTEMATIC DELETIONS AND INSERTIONS 15.5 Linker - insertion Mutagenesis 15.5 GENERATION OF LINKER - INSERTION MUTANTS WITH FREQUENTLY CUTTING RESTRICTION ENZYMES 15.8 ...
Page 15-18
... deletions , but also groups of deletions whose endpoints are tightly clustered . • The entire series of enzymatic reactions ( exonuclease digestion , treatment with nuclease S1 or mung - bean nuclease and DNA polymerase , and ...
... deletions , but also groups of deletions whose endpoints are tightly clustered . • The entire series of enzymatic reactions ( exonuclease digestion , treatment with nuclease S1 or mung - bean nuclease and DNA polymerase , and ...
Page 15-33
... deletions must be constructed , sequenced , and matched . For example , in the original description of the method ( McKnight and Kingsbury 1982 ) , 43 5 ' deletions and 42 3 ' deletions were constructed to obtain 15 perfectly matched ...
... deletions must be constructed , sequenced , and matched . For example , in the original description of the method ( McKnight and Kingsbury 1982 ) , 43 5 ' deletions and 42 3 ' deletions were constructed to obtain 15 perfectly matched ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml