Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-55
... described below . The protocol actually measures the temperature at which dissociation of the double- stranded DNA becomes irreversible ( T ; ) in nonequilibrium conditions that do not favor rehybridization of the released probe to the ...
... described below . The protocol actually measures the temperature at which dissociation of the double- stranded DNA becomes irreversible ( T ; ) in nonequilibrium conditions that do not favor rehybridization of the released probe to the ...
Page 12-34
... described on pages 12.16-12.17 , steps 1-8 . The filters are then processed and screened as described below . The following method was provided by S. McKnight . 1. Remove the nitrocellulose filters from the lawn of plaques with blunt ...
... described on pages 12.16-12.17 , steps 1-8 . The filters are then processed and screened as described below . The following method was provided by S. McKnight . 1. Remove the nitrocellulose filters from the lawn of plaques with blunt ...
Page 15-72
... described in detail in Chapter 11. However , because putative positive colonies almost certainly contain a mixture of wild - type and mutated molecules , it is necessary to carry out a second round of transformation and screening as ...
... described in detail in Chapter 11. However , because putative positive colonies almost certainly contain a mixture of wild - type and mutated molecules , it is necessary to carry out a second round of transformation and screening as ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml