Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 39
Page 10-7
... dithiothreitol 500 μg / ml bovine serum albumin ( Fraction V ; Sigma ) Divide the stock solution into small aliquots and store them at −20 ° C . 10 × Ligation buffer 0.5 M Tris Cl ( pH 7.6 ) 100 mM MgCl , 100 mм dithiothreitol 500 μg ...
... dithiothreitol 500 μg / ml bovine serum albumin ( Fraction V ; Sigma ) Divide the stock solution into small aliquots and store them at −20 ° C . 10 × Ligation buffer 0.5 M Tris Cl ( pH 7.6 ) 100 mM MgCl , 100 mм dithiothreitol 500 μg ...
Page 12-34
... dithiothreitol to a final concentration of 1 mM . 10 × Binding buffer 250 mM HEPES ( pH 7.9 ) 30 mM MgCl2 40 mM KCI 5. Decant the second batch of denaturation solution into a graduated cylin- der . Dilute the solution with an equal ...
... dithiothreitol to a final concentration of 1 mM . 10 × Binding buffer 250 mM HEPES ( pH 7.9 ) 30 mM MgCl2 40 mM KCI 5. Decant the second batch of denaturation solution into a graduated cylin- der . Dilute the solution with an equal ...
Page 15-29
... dithiothreitol The use of two different DNA polymerases in the repair reaction results in an approximately threefold increase in recovery of mutants . 11. Add 70 μl of TE ( pH 7.6 ) , and then extract the sample once with an equal ...
... dithiothreitol The use of two different DNA polymerases in the repair reaction results in an approximately threefold increase in recovery of mutants . 11. Add 70 μl of TE ( pH 7.6 ) , and then extract the sample once with an equal ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
82 other sections not shown
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml