Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-18
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single ... DNA . For exam- ple , when using single - stranded DNA probes in nuclease ... double - stranded DNA templates into radiolabeled RNA ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single ... DNA . For exam- ple , when using single - stranded DNA probes in nuclease ... double - stranded DNA templates into radiolabeled RNA ...
Page 14-28
... double - stranded DNA , which accumulates at an approximately exponential rate . After 12-15 cycles of amplification , the concentration of one of the primers becomes limiting , and the rate of production of double - stranded DNA ...
... double - stranded DNA , which accumulates at an approximately exponential rate . After 12-15 cycles of amplification , the concentration of one of the primers becomes limiting , and the rate of production of double - stranded DNA ...
Page 15-20
... double- stranded DNA is given by the equation : dM -2V M. t = m n dt [ Km + ( S ) , ] m n m where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , Vm = maximum reaction velocity ( expressed as moles ...
... double- stranded DNA is given by the equation : dM -2V M. t = m n dt [ Km + ( S ) , ] m n m where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , Vm = maximum reaction velocity ( expressed as moles ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml