Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-55
... ethanol bath ) for 15 minutes or to store it for more prolonged periods at -20 ° C before centrifugation . In our hands , these procedures do not improve the efficiency with which DNA is precipitated by ethanol . • Save the ethanol ...
... ethanol bath ) for 15 minutes or to store it for more prolonged periods at -20 ° C before centrifugation . In our hands , these procedures do not improve the efficiency with which DNA is precipitated by ethanol . • Save the ethanol ...
Page 9-22
... ethanol at room temperature into each of a series of disposable 50 - ml polypropylene centrifuge tubes . Using wide - bore pi- pettes , carefully layer the cell suspensions under the ethanol . 5. Recover the DNA from each tube by slowly ...
... ethanol at room temperature into each of a series of disposable 50 - ml polypropylene centrifuge tubes . Using wide - bore pi- pettes , carefully layer the cell suspensions under the ethanol . 5. Recover the DNA from each tube by slowly ...
Page 13-98
... ethanol precipitation e . Poor suppression of Ts in the C ladder 2. Ts are weak in the C + T ladder . 3. Bands are present in C and / or C + T ladders at all guanine positions , not just those where guanine follows a pyrimi- dine in the ...
... ethanol precipitation e . Poor suppression of Ts in the C ladder 2. Ts are weak in the C + T ladder . 3. Bands are present in C and / or C + T ladders at all guanine positions , not just those where guanine follows a pyrimi- dine in the ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml