Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-14
... exonuclease activity , so that the radioactive product is synthesized exclusively by primer extension rather than by nick translation and is not degraded exonucleolytically . The reaction is carried out at pH 6.6 , where the 3'5 ' ...
... exonuclease activity , so that the radioactive product is synthesized exclusively by primer extension rather than by nick translation and is not degraded exonucleolytically . The reaction is carried out at pH 6.6 , where the 3'5 ' ...
Page 10-56
... exonuclease activity of bacteriophage T4 DNA polymerase first digests double - stranded DNA to produce molecules with recessed 3 ' termini . On subsequent addition of radiolabeled dNTPs , the partially digested DNA molecules serve as ...
... exonuclease activity of bacteriophage T4 DNA polymerase first digests double - stranded DNA to produce molecules with recessed 3 ' termini . On subsequent addition of radiolabeled dNTPs , the partially digested DNA molecules serve as ...
Page 13-9
... exonuclease activity . Sequenase version 2.0 is a genetically engineered form of Sequenase that entirely lacks 3'5 ' exonuclease activity , is extremely stable , and has a threefold higher specific activity than the chemically modified ...
... exonuclease activity . Sequenase version 2.0 is a genetically engineered form of Sequenase that entirely lacks 3'5 ' exonuclease activity , is extremely stable , and has a threefold higher specific activity than the chemically modified ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml