Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-7
... DNA The first generation of bacteriophage vectors , such as Charon 4A ( see Chapter 2 , page 2.19 ) and Agt - WES ( see Maniatis et al . 1982 for map ) , which were developed in the late 1970s , could accept segments of foreign DNA ...
... DNA The first generation of bacteriophage vectors , such as Charon 4A ( see Chapter 2 , page 2.19 ) and Agt - WES ( see Maniatis et al . 1982 for map ) , which were developed in the late 1970s , could accept segments of foreign DNA ...
Page 9-8
... foreign DNA and does not yield a high proportion of nonrecombinant bacteriophages . The arms of many vectors are available from commercial sources , and it may be cost - effective for those planning to make only one genomic DNA library ...
... foreign DNA and does not yield a high proportion of nonrecombinant bacteriophages . The arms of many vectors are available from commercial sources , and it may be cost - effective for those planning to make only one genomic DNA library ...
Page 10-29
... foreign DNA sequences can usually be inserted by directional cloning no further than 30-40 nucleotides downstream from the position at which RNA synthesis is initiated in vitro . In many cases , the polycloning site also provides a ...
... foreign DNA sequences can usually be inserted by directional cloning no further than 30-40 nucleotides downstream from the position at which RNA synthesis is initiated in vitro . In many cases , the polycloning site also provides a ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml