Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-2
... DNA to the elucidation of the complete sequence of the human genome . Although this rapid rate of progress has been ... fragments of mammalian genomic DNA . Before then , genomic libraries consisted of fragments of DNA obtained by ...
... DNA to the elucidation of the complete sequence of the human genome . Although this rapid rate of progress has been ... fragments of mammalian genomic DNA . Before then , genomic libraries consisted of fragments of DNA obtained by ...
Page 9-32
... DNA BY AGAROSE GEL ELECTROPHORESIS 1. Digest an appropriate amount of DNA with one or more restriction enzymes . For Southern analysis ... DNA SEPARATION OF RESTRICTION FRAGMENTS OF MAMMALIAN GENOMIC DNA BY AGAROSE GEL ELECTROPHORESIS 9 32.
... DNA BY AGAROSE GEL ELECTROPHORESIS 1. Digest an appropriate amount of DNA with one or more restriction enzymes . For Southern analysis ... DNA SEPARATION OF RESTRICTION FRAGMENTS OF MAMMALIAN GENOMIC DNA BY AGAROSE GEL ELECTROPHORESIS 9 32.
Page 9-38
... DNA TO NITROCELLULOSE FILTERS 1. After electrophoresis , transfer the gel to a glass baking dish and trim away any ... fragments of interest are larger than approximately 15 kb , transfer may be improved by nicking the DNA by brief ...
... DNA TO NITROCELLULOSE FILTERS 1. After electrophoresis , transfer the gel to a glass baking dish and trim away any ... fragments of interest are larger than approximately 15 kb , transfer may be improved by nicking the DNA by brief ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml