Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxvii
... Fusion Proteins 17.3 Vector Systems for the Expression of lacZ Fusion Genes 17.3 Construction of pUR - cDNA Plasmids and Detection of Fusion Proteins 17.7 Preparation of Fusion Proteins for Antibody Production 17.8 Production of Intact ...
... Fusion Proteins 17.3 Vector Systems for the Expression of lacZ Fusion Genes 17.3 Construction of pUR - cDNA Plasmids and Detection of Fusion Proteins 17.7 Preparation of Fusion Proteins for Antibody Production 17.8 Production of Intact ...
Page 12-8
... fusion proteins than those with insertions at the 5 ' terminus ( Stanley 1983 ) . In at least some cases , these fusion proteins retain ß - galactosidase activity ( Rüther and Müller - Hill 1983 ) , which can serve as a useful marker ...
... fusion proteins than those with insertions at the 5 ' terminus ( Stanley 1983 ) . In at least some cases , these fusion proteins retain ß - galactosidase activity ( Rüther and Müller - Hill 1983 ) , which can serve as a useful marker ...
Page 12-15
... proteins whose genes are cloned by immunological screening . • Demonstration that the fusion protein expressed by the clone can block the interaction between the purified protein of interest and a specific an- tibody . For example , the ...
... proteins whose genes are cloned by immunological screening . • Demonstration that the fusion protein expressed by the clone can block the interaction between the purified protein of interest and a specific an- tibody . For example , the ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml