Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiii
A Laboratory Manual Joseph Sambrook, Tom Maniatis. 6 Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 ... GELS 6.49 L PULSED - FIELD GEL ELECTROPHORESIS 6.50 Design of the Contents xiii.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. 6 Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 ... GELS 6.49 L PULSED - FIELD GEL ELECTROPHORESIS 6.50 Design of the Contents xiii.
Page xiv
... Gel Electrophoresis 6.52 Preparation of DNA for Pulsed - field Gel Electrophoresis 6.53 ISOLATION OF INTACT DNA FROM MAMMALIAN CELLS 6.53 ISOLATION OF INTACT DNA FROM YEAST 6.53 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 ...
... Gel Electrophoresis 6.52 Preparation of DNA for Pulsed - field Gel Electrophoresis 6.53 ISOLATION OF INTACT DNA FROM MAMMALIAN CELLS 6.53 ISOLATION OF INTACT DNA FROM YEAST 6.53 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 ...
Page 15-36
... gel electrophoresis and recloned into an appropriate plasmid vector . Because this fractionation step is at best accurate only to ± 5 nucleotide pairs , plasmids whose lengths are exactly the same as that of the original recombi- nant ...
... gel electrophoresis and recloned into an appropriate plasmid vector . Because this fractionation step is at best accurate only to ± 5 nucleotide pairs , plasmids whose lengths are exactly the same as that of the original recombi- nant ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml