Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 69
Page 8-86
... Genetic engineering ( ed . R. William- son ) , vol . 1 , p . 1. Academic Press , London . Williams , J.G. and M.M. ... genes into an E. coli plasmid . Nucleic Acids Res . 3 : 1961 . Wood , W.I. , D.J. Capon , C.C. Simonsen , D.L. Eaton ...
... Genetic engineering ( ed . R. William- son ) , vol . 1 , p . 1. Academic Press , London . Williams , J.G. and M.M. ... genes into an E. coli plasmid . Nucleic Acids Res . 3 : 1961 . Wood , W.I. , D.J. Capon , C.C. Simonsen , D.L. Eaton ...
Page 10-38
... genes that are developmentally regulated and genes that are specifically expressed in particular types of differentiated cells . The identifi- cation of cDNA clones may be accomplished by a number of methods , all of which are capable ...
... genes that are developmentally regulated and genes that are specifically expressed in particular types of differentiated cells . The identifi- cation of cDNA clones may be accomplished by a number of methods , all of which are capable ...
Page 11-5
... genetic code , from short tracts of amino acid sequence determined by the sequencing of small quantities of highly purified proteins ( see , e.g. , Hewick et al . 1981 ; Hunkapil- ler et al . 1983 ; Kent et al . 1987 ) . Because of the ...
... genetic code , from short tracts of amino acid sequence determined by the sequencing of small quantities of highly purified proteins ( see , e.g. , Hewick et al . 1981 ; Hunkapil- ler et al . 1983 ; Kent et al . 1987 ) . Because of the ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml