Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-2
... genomic DNA libraries are now increasingly viewed as matrices that can be assembled into maps of the genome from ... genomic DNA . Before then , genomic libraries consisted of fragments of DNA obtained by complete digestion of genomic ...
... genomic DNA libraries are now increasingly viewed as matrices that can be assembled into maps of the genome from ... genomic DNA . Before then , genomic libraries consisted of fragments of DNA obtained by complete digestion of genomic ...
Page 9-31
... genomic DNA is usually accom- plished by the transfer techniques described by Southern ( 1975 ) . Genomic DNA is digested with one or more restriction enzymes , and the resulting fragments are separated according to size by ...
... genomic DNA is usually accom- plished by the transfer techniques described by Southern ( 1975 ) . Genomic DNA is digested with one or more restriction enzymes , and the resulting fragments are separated according to size by ...
Page 12-6
... genomes contain few introns , there is little to be gained by constructing cDNA libraries in these vectors . Instead , randomly sheared genomic DNA can be used to create a library ( for details , see Chapter 9 ) in which all coding ...
... genomes contain few introns , there is little to be gained by constructing cDNA libraries in these vectors . Instead , randomly sheared genomic DNA can be used to create a library ( for details , see Chapter 9 ) in which all coding ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml