Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-37
... host hflA gene and the cIII gene product . Mutation of the hflA gene enhances the stability of the cII gene product ... hosts , recombination can in theory result in rearrangement or loss of cDNA sequences . Fortunately , only a small ...
... host hflA gene and the cIII gene product . Mutation of the hflA gene enhances the stability of the cII gene product ... hosts , recombination can in theory result in rearrangement or loss of cDNA sequences . Fortunately , only a small ...
Page 8-38
... host and bacteriophage genomes ; supF suppresses the amber mutation in the lysis ( S ) gene of the bacteriophage and allows Agt11 to lyse the infected cells ; plasmid pMC9 expresses high levels of the lac repressor ( the product of the ...
... host and bacteriophage genomes ; supF suppresses the amber mutation in the lysis ( S ) gene of the bacteriophage and allows Agt11 to lyse the infected cells ; plasmid pMC9 expresses high levels of the lac repressor ( the product of the ...
Page 9-8
... host - encoded recBC nuclease ( Friedman and Hays 1986 ) . In recA bacteria , therefore , the rolling - circle DNA ... hosts reduces the likelihood of rearrangement or loss of foreign DNA sequences by recombination . Particularly ...
... host - encoded recBC nuclease ( Friedman and Hays 1986 ) . In recA bacteria , therefore , the rolling - circle DNA ... hosts reduces the likelihood of rearrangement or loss of foreign DNA sequences by recombination . Particularly ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml