Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-44
... infected bacteria or induced lysogens , essentially as described for Agt11 . In addition , AZAP fusion proteins may be expressed from high - copy - number plasmids following the excision of the DNA insert from the bacteriophage vector ...
... infected bacteria or induced lysogens , essentially as described for Agt11 . In addition , AZAP fusion proteins may be expressed from high - copy - number plasmids following the excision of the DNA insert from the bacteriophage vector ...
Page 8-79
... infect a restriction- competent strain of E. coli . During amplification , it is important to suppress the production ... infected culture on a 150 - mm petri dish containing NZCYM agar . Incubate for 12 hours at 42 ° C ( to prevent the ...
... infect a restriction- competent strain of E. coli . During amplification , it is important to suppress the production ... infected culture on a 150 - mm petri dish containing NZCYM agar . Incubate for 12 hours at 42 ° C ( to prevent the ...
Page 15-76
... infect the dut ung F strain of E. coli . Adjust the volume of the inoculum so as to achieve a multiplicity of infection of 0.1 pfu / bacterial cell . Grow the infected culture for 6 hours as described . • Incubate the infected cultures ...
... infect the dut ung F strain of E. coli . Adjust the volume of the inoculum so as to achieve a multiplicity of infection of 0.1 pfu / bacterial cell . Grow the infected culture for 6 hours as described . • Incubate the infected cultures ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml