Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-8
... length ( which for screening of a typical mammalian cDNA library is 17-18 nucleotides [ Lathe 1985 ] ) , it is worthwhile increasing its length in order to maximize its specificity . The concomitant increase in mismatches that occurs as ...
... length ( which for screening of a typical mammalian cDNA library is 17-18 nucleotides [ Lathe 1985 ] ) , it is worthwhile increasing its length in order to maximize its specificity . The concomitant increase in mismatches that occurs as ...
Page 13-45
... length of the gel . A length of 40-50 cm is standard , but apparatuses that accommodate gels up to 100 cm in length are available . However , long gels are extremely difficult to handle and require special , large containers for ...
... length of the gel . A length of 40-50 cm is standard , but apparatuses that accommodate gels up to 100 cm in length are available . However , long gels are extremely difficult to handle and require special , large containers for ...
Page 15-89
... length linear mole- cules . 5. Purify the full - length linear DNA by preparative agarose gel elec- trophoresis using one of the methods described in Chapter 6. Redissolve the DNA in TE ( pH 7.6 ) at a concentration of 250 μg / ml ...
... length linear mole- cules . 5. Purify the full - length linear DNA by preparative agarose gel elec- trophoresis using one of the methods described in Chapter 6. Redissolve the DNA in TE ( pH 7.6 ) at a concentration of 250 μg / ml ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml