Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 48
Page 13-34
... linear DNA by liberating mononucleotides from the 3 ' termini and a weaker single - strand - specific endonucleolytic activity . Thus , the products of digestion of double - stranded DNA with BAL 31 are truncated molecules carrying ...
... linear DNA by liberating mononucleotides from the 3 ' termini and a weaker single - strand - specific endonucleolytic activity . Thus , the products of digestion of double - stranded DNA with BAL 31 are truncated molecules carrying ...
Page 15-9
... linear DNA will be used as an electrophoretic marker ( see h ) . g . After the eight test reactions have incubated for 30 minutes , quickly transfer the tubes to an ice - water bath . Add 5 μl of 50 mм EDTA ( pH 8.0 ) to each tube to ...
... linear DNA will be used as an electrophoretic marker ( see h ) . g . After the eight test reactions have incubated for 30 minutes , quickly transfer the tubes to an ice - water bath . Add 5 μl of 50 mм EDTA ( pH 8.0 ) to each tube to ...
Page 15-89
... linear DNA and considerably faster than relaxed circular DNA . d . Examine the gel by ultraviolet illumination , and determine the condi- tions that give the maximum yield of full - length linear molecules . Usually not more than 33 ...
... linear DNA and considerably faster than relaxed circular DNA . d . Examine the gel by ultraviolet illumination , and determine the condi- tions that give the maximum yield of full - length linear molecules . Usually not more than 33 ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
82 other sections not shown
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml