Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxvi
... Mammalian Cells Expression of Proteins 16.3 EXPRESSION OF PROTEINS FROM CLONED GENES 16.3 FUNCTIONAL COMPONENTS OF MAMMALIAN EXPRESSION VECTORS 16.5 Prokaryotic Plasmid Sequences That Facilitate the Construction , Propagation , and ...
... Mammalian Cells Expression of Proteins 16.3 EXPRESSION OF PROTEINS FROM CLONED GENES 16.3 FUNCTIONAL COMPONENTS OF MAMMALIAN EXPRESSION VECTORS 16.5 Prokaryotic Plasmid Sequences That Facilitate the Construction , Propagation , and ...
Page 9-2
... mammalian genomic DNA . Before then , genomic libraries consisted of fragments of DNA obtained by complete digestion of genomic DNA with a restriction enzyme such as EcoRI . The earliest libraries of mammalian genomic DNA contained only ...
... mammalian genomic DNA . Before then , genomic libraries consisted of fragments of DNA obtained by complete digestion of genomic DNA with a restriction enzyme such as EcoRI . The earliest libraries of mammalian genomic DNA contained only ...
Page 9-32
... mammalian genomic DNA , approxi- mately 10 μg of DNA should be loaded into each slot of the gel when probes of standard length ( > 500 bp ) and high specific activity ( > 109 cpm / μg ) are used to detect single - copy sequences ; 30-50 ...
... mammalian genomic DNA , approxi- mately 10 μg of DNA should be loaded into each slot of the gel when probes of standard length ( > 500 bp ) and high specific activity ( > 109 cpm / μg ) are used to detect single - copy sequences ; 30-50 ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml