Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-11
... METHOD Unlike the chain - termination technique , which involves synthesis , the Maxam - Gilbert method involves chemical degradation of the original DNA . This method grew out of studies of the interaction between the lac repressor and ...
... METHOD Unlike the chain - termination technique , which involves synthesis , the Maxam - Gilbert method involves chemical degradation of the original DNA . This method grew out of studies of the interaction between the lac repressor and ...
Page 14-10
... method or , more commonly , by the Sanger dideoxy - mediated chain- termination method ( see Chapter 13 ) . Three types of polymerase chain reaction are used to generate templates for sequencing by the Sanger method : • A conventional ...
... method or , more commonly , by the Sanger dideoxy - mediated chain- termination method ( see Chapter 13 ) . Three types of polymerase chain reaction are used to generate templates for sequencing by the Sanger method : • A conventional ...
Page 15-62
... method is much lower than the number obtained by more traditional mutagenesis procedures , the proportion of plaques that are generated by bacteriophages carrying the desired mutation is much greater . Kunkel's method is therefore ...
... method is much lower than the number obtained by more traditional mutagenesis procedures , the proportion of plaques that are generated by bacteriophages carrying the desired mutation is much greater . Kunkel's method is therefore ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml