Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 9
Page xii
... METHYLASE dcm METHYLASE 5.15 5.15 METHYLATION - DEPENDENT RESTRICTION SYSTEMS IN E. coli M.EcoRI METHYLASE 5.15 Modification of Restriction Sites by DNA Methylation 5.16 Influence of Methylation on DNA Mapping 5.26 DIGESTING DNA WITH ...
... METHYLASE dcm METHYLASE 5.15 5.15 METHYLATION - DEPENDENT RESTRICTION SYSTEMS IN E. coli M.EcoRI METHYLASE 5.15 Modification of Restriction Sites by DNA Methylation 5.16 Influence of Methylation on DNA Mapping 5.26 DIGESTING DNA WITH ...
Page 8-39
... methylases to protect potential sites of cleavage with the restriction enzymes BamHI and HindIII . Because M.HindIII methylase is not commercially available , the cDNA is methylated with M.BamHI methylase and M.AluI methylase ( which ...
... methylases to protect potential sites of cleavage with the restriction enzymes BamHI and HindIII . Because M.HindIII methylase is not commercially available , the cDNA is methylated with M.BamHI methylase and M.AluI methylase ( which ...
Page 8-66
... methylase is inhibited by Mg ** ions . L [ [ [ 2. Remove two aliquots ( 2 μl each ) and place in small ( 0.5 - ml ) microfuge tubes . Number these aliquots 1 and 2. Store at 0 ° C . [ It is important to use small microfuge tubes to ...
... methylase is inhibited by Mg ** ions . L [ [ [ 2. Remove two aliquots ( 2 μl each ) and place in small ( 0.5 - ml ) microfuge tubes . Number these aliquots 1 and 2. Store at 0 ° C . [ It is important to use small microfuge tubes to ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml