Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 56
Page 11-36
... tube in a dry - ice / methanol bath until the mixture is frozen . Remove the tube from the bath , and allow the mixture to thaw at room temperature . 4. Centrifuge at 12,000g for 5 minutes at 4 ° C in a microfuge . Using a pasteur ...
... tube in a dry - ice / methanol bath until the mixture is frozen . Remove the tube from the bath , and allow the mixture to thaw at room temperature . 4. Centrifuge at 12,000g for 5 minutes at 4 ° C in a microfuge . Using a pasteur ...
Page 13-26
... microfuge tube . Store this tube on ice . Between bursts , centrifuge the original mi- crofuge tube for 2 seconds to deposit the DNA solution at the bottom of the tube , and add fresh ice to the sonicator horn to maintain the ...
... microfuge tube . Store this tube on ice . Between bursts , centrifuge the original mi- crofuge tube for 2 seconds to deposit the DNA solution at the bottom of the tube , and add fresh ice to the sonicator horn to maintain the ...
Page 13-61
... microfuge tube , and then add 2 μl of single - stranded DNA template . Close the top of the tube , and incubate it for 30 minutes at 55 ° C . The concentration of single - stranded DNA in small - scale preparations of bac- teriophage ...
... microfuge tube , and then add 2 μl of single - stranded DNA template . Close the top of the tube , and incubate it for 30 minutes at 55 ° C . The concentration of single - stranded DNA in small - scale preparations of bac- teriophage ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml