Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-23
... molecules . The reaction often proceeds asynchronously , so that different termini of DNA molecules carry homopolymeric tails of very different lengths . This prob- lem can be alleviated , but not completely solved , by using plasmids ...
... molecules . The reaction often proceeds asynchronously , so that different termini of DNA molecules carry homopolymeric tails of very different lengths . This prob- lem can be alleviated , but not completely solved , by using plasmids ...
Page 15-27
... molecules . Few of the molecules are cleaved more than once by the enzyme . Digestion with a restriction enzyme that cleaves at one end of the target sequence therefore generates a mixed population that contains full - length linear ...
... molecules . Few of the molecules are cleaved more than once by the enzyme . Digestion with a restriction enzyme that cleaves at one end of the target sequence therefore generates a mixed population that contains full - length linear ...
Page 15-39
... molecules , which migrate much slower through agarose gels than closed circular DNAS of the same size . There should be no increase in the ratio of relaxed circular molecules to super- helical molecules in the control samples . Pool the ...
... molecules , which migrate much slower through agarose gels than closed circular DNAS of the same size . There should be no increase in the ratio of relaxed circular molecules to super- helical molecules in the control samples . Pool the ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml