Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 15-61
... mutations at these sites . Thus , only a fraction of the plaques that hybridize to the oligonucleotide probe may actually carry the desired mutation . • The nature of the vector . Oligonucleotide - mediated mutagenesis is general- ly ...
... mutations at these sites . Thus , only a fraction of the plaques that hybridize to the oligonucleotide probe may actually carry the desired mutation . • The nature of the vector . Oligonucleotide - mediated mutagenesis is general- ly ...
Page 15-81
... mutations that affect the function of proteins such as the human LDL receptor ( Davis et al . 1986 ) and factor VIII ( Gitschier et al . 1988 ) . However , this method also has its own set of restrictions : The mutations are sometimes ...
... mutations that affect the function of proteins such as the human LDL receptor ( Davis et al . 1986 ) and factor VIII ( Gitschier et al . 1988 ) . However , this method also has its own set of restrictions : The mutations are sometimes ...
Page 15-112
... mutations in vitro . Proc . Natl . Acad . Sci . 79 : 1588 . Smith , M. 1985. In vitro mutagenesis . Annu . Rev. Genet . 19 : 423 . Solnick , D. 1981. An adenovirus mutant defective in splicing RNA from early region 1A . Nature 291 : 508 ...
... mutations in vitro . Proc . Natl . Acad . Sci . 79 : 1588 . Smith , M. 1985. In vitro mutagenesis . Annu . Rev. Genet . 19 : 423 . Solnick , D. 1981. An adenovirus mutant defective in splicing RNA from early region 1A . Nature 291 : 508 ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml