Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-7
... nucleotides would therefore be expected to be represented only once in the genome . However , the distribution of nucleotides in the coding sequences of mammalian genomes is nonrandom ( Lathe 1985 ) , and it is therefore advisable to ...
... nucleotides would therefore be expected to be represented only once in the genome . However , the distribution of nucleotides in the coding sequences of mammalian genomes is nonrandom ( Lathe 1985 ) , and it is therefore advisable to ...
Page 13-45
... nucleotides of the 5 ' terminus of the primer ( or within 50 nucleotides of the radiolabeled terminus in the case of Maxam - Gilbert sequencing ) , gels should be cast with high ( 12-20 % ) concentrations of acrylamide . Sequences lying ...
... nucleotides of the 5 ' terminus of the primer ( or within 50 nucleotides of the radiolabeled terminus in the case of Maxam - Gilbert sequencing ) , gels should be cast with high ( 12-20 % ) concentrations of acrylamide . Sequences lying ...
Page 15-55
... nucleotides are required at the 3 ' terminus of the mutagenic oligonu- cleotide . • The difference in thermal ... nucleotides in length , with the mismatch centrally located . 2. Oligonucleotides used to create deletions or insertions or ...
... nucleotides are required at the 3 ' terminus of the mutagenic oligonu- cleotide . • The difference in thermal ... nucleotides in length , with the mismatch centrally located . 2. Oligonucleotides used to create deletions or insertions or ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml