Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xviii
... Nylon Membranes 9.42 CAPILLARY TRANSFER OF DNA TO NYLON MEMBRANES UNDER NEUTRAL CONDITIONS 9.44 CAPILLARY TRANSFER OF DNA TO NYLON MEMBRANES UNDER ALKALINE CONDITIONS 9.45 HYBRIDIZATION OF RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ...
... Nylon Membranes 9.42 CAPILLARY TRANSFER OF DNA TO NYLON MEMBRANES UNDER NEUTRAL CONDITIONS 9.44 CAPILLARY TRANSFER OF DNA TO NYLON MEMBRANES UNDER ALKALINE CONDITIONS 9.45 HYBRIDIZATION OF RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ...
Page 9-42
... Nylon Membranes For almost 20 years , immobilization and hybridization of denatured DNA was carried out exclusively with nitrocellulose - first with powder ( Hall and Spiegelman 1961 ) and then with sheets ( Nygaard ... Nylon Membranes 9 42.
... Nylon Membranes For almost 20 years , immobilization and hybridization of denatured DNA was carried out exclusively with nitrocellulose - first with powder ( Hall and Spiegelman 1961 ) and then with sheets ( Nygaard ... Nylon Membranes 9 42.
Page 9-44
... NYLON MEMBRANES UNDER NEUTRAL CONDITIONS 1. Fractionate the DNA by gel electrophoresis , and process the gel as described in steps 1-3 on page 9.38 . 2. While the gel is in the neutralization solution ... NYLON MEMBRANES UNDER NEUTRAL.
... NYLON MEMBRANES UNDER NEUTRAL CONDITIONS 1. Fractionate the DNA by gel electrophoresis , and process the gel as described in steps 1-3 on page 9.38 . 2. While the gel is in the neutralization solution ... NYLON MEMBRANES UNDER NEUTRAL.
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml