Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-21
... oligonucleotide ( usually 1-10 nmoles ) in 1 ml of sterile , filtered H2O ( Milli - Q or equivalent ) . Vortex ... Oligonucleotide Probes 11.21 PURIFICATION OF SYNTHETIC OLIGONUCLEOTIDES 11 21 Preparation of Synthetic Oligonucleotides 11 21.
... oligonucleotide ( usually 1-10 nmoles ) in 1 ml of sterile , filtered H2O ( Milli - Q or equivalent ) . Vortex ... Oligonucleotide Probes 11.21 PURIFICATION OF SYNTHETIC OLIGONUCLEOTIDES 11 21 Preparation of Synthetic Oligonucleotides 11 21.
Page 11-30
... oligonucleotide applied to the column should elute in the first fraction . 5. Evaporate ... oligonucleotide LABELING OF SYNTHETIC OLIGONUCLEOTIDES BY PHOSPHORYLATION WITH BACTERIOPHAGE T4 POLYNUCLEOTIDE 11.30 Synthetic Oligonucleotide Probes.
... oligonucleotide applied to the column should elute in the first fraction . 5. Evaporate ... oligonucleotide LABELING OF SYNTHETIC OLIGONUCLEOTIDES BY PHOSPHORYLATION WITH BACTERIOPHAGE T4 POLYNUCLEOTIDE 11.30 Synthetic Oligonucleotide Probes.
Page 11-31
... oligonucleotide to high specific activity . Labeling of different amounts of oligonucleotide can easily be achieved by increasing or decreasing the size of the reaction , keeping the concentrations of all components constant . 1. Set up ...
... oligonucleotide to high specific activity . Labeling of different amounts of oligonucleotide can easily be achieved by increasing or decreasing the size of the reaction , keeping the concentrations of all components constant . 1. Set up ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml