Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-33
... ( phagemid vectors ) or transfect ( bacteriophage M13 vectors ; see Chapter 4 for test ligations ) competent E. coli of the appropriate strain . Plate the transfected or transformed bacteria on media containing IPTG or X - gal . The ...
... ( phagemid vectors ) or transfect ( bacteriophage M13 vectors ; see Chapter 4 for test ligations ) competent E. coli of the appropriate strain . Plate the transfected or transformed bacteria on media containing IPTG or X - gal . The ...
Page 15-61
... phagemid clone to another . Although the phagemid system can generally be used to isolate simple mutants ( containing point mutations and small deletions or insertions ) , bacteriophage M13 vectors are preferred when constructing more ...
... phagemid clone to another . Although the phagemid system can generally be used to isolate simple mutants ( containing point mutations and small deletions or insertions ) , bacteriophage M13 vectors are preferred when constructing more ...
Page 15-72
... phagemid DNA from each of the cultures by one of the methods described in Chapter 1 . • Transform competent E. coli ( strain MV1184 ) with each of the preparations of phagemid DNA . · Prepare single - stranded phagemid DNA by ...
... phagemid DNA from each of the cultures by one of the methods described in Chapter 1 . • Transform competent E. coli ( strain MV1184 ) with each of the preparations of phagemid DNA . · Prepare single - stranded phagemid DNA by ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml