Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxvi
... PHOSPHATE AND DNA 16.32 Standard Protocol for Calcium Phosphate - mediated Transfection of Adherent Cells 16.33 Calcium Phosphate - mediated Transfection of Adherent Cells in Suspension 16.37 Calcium Phosphate - mediated Transfection of ...
... PHOSPHATE AND DNA 16.32 Standard Protocol for Calcium Phosphate - mediated Transfection of Adherent Cells 16.33 Calcium Phosphate - mediated Transfection of Adherent Cells in Suspension 16.37 Calcium Phosphate - mediated Transfection of ...
Page 11-32
... phosphate will migrate in the same direction as the solvent ( inorganic phosphate migrates slightly slower than the solvent front and ATP is approximately equidistant between the origin and the inorganic phosphate ) . Thus , the ...
... phosphate will migrate in the same direction as the solvent ( inorganic phosphate migrates slightly slower than the solvent front and ATP is approximately equidistant between the origin and the inorganic phosphate ) . Thus , the ...
Page 15-67
... phosphate will migrate in the same direction as the solvent ( inorganic phosphate migrates slightly slower than the solvent front and ATP is approximately equidistant between the origin and the inorganic phosphate ) . Thus , the ...
... phosphate will migrate in the same direction as the solvent ( inorganic phosphate migrates slightly slower than the solvent front and ATP is approximately equidistant between the origin and the inorganic phosphate ) . Thus , the ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml