Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 35
Page 8-70
... pipette . b . Attach a piece of sterile polyvinyl chloride tubing ( of the type normally used in peristaltic pumps ) to the narrow end of the pipette . Dip the wide end into a solution of TE ( pH 7.6 ) containing 0.1 M NaCl in a beaker ...
... pipette . b . Attach a piece of sterile polyvinyl chloride tubing ( of the type normally used in peristaltic pumps ) to the narrow end of the pipette . Dip the wide end into a solution of TE ( pH 7.6 ) containing 0.1 M NaCl in a beaker ...
Page 9-18
... pipette very slowly to avoid disturbing the material at the interface . If the DNA solution is so viscous that it cannot easily be drawn into a wide - bore pipette , use a long pipette attached to a water - suction vacuum pump to remove ...
... pipette very slowly to avoid disturbing the material at the interface . If the DNA solution is so viscous that it cannot easily be drawn into a wide - bore pipette , use a long pipette attached to a water - suction vacuum pump to remove ...
Page 9-22
... pipette whose end has been sealed and bent into a U shape . The DNA will adhere to the pasteur pipette , forming a gelatinous mass . Continue stirring until the ethanol and the aqueous phase are thoroughly mixed . 6. Transfer each ...
... pipette whose end has been sealed and bent into a U shape . The DNA will adhere to the pasteur pipette , forming a gelatinous mass . Continue stirring until the ethanol and the aqueous phase are thoroughly mixed . 6. Transfer each ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml