Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 53
Page 8-73
... plaques on strains of E. coli such as Y1090hsdR in the presence of the inducer IPTG and the chromogenic substrate X - gal ; recombinant plaques are colorless . Nonrecombinant bacteriophages do not present a problem when bac- teriophages ...
... plaques on strains of E. coli such as Y1090hsdR in the presence of the inducer IPTG and the chromogenic substrate X - gal ; recombinant plaques are colorless . Nonrecombinant bacteriophages do not present a problem when bac- teriophages ...
Page 13-33
... plaques ) . Bacterial colonies carrying phagemids can be stored at 4 ° C for a few weeks on the original agar plates . However , recombinant bacteriophage M13 plaques should be picked as soon as possible , since the bacteriophage ...
... plaques ) . Bacterial colonies carrying phagemids can be stored at 4 ° C for a few weeks on the original agar plates . However , recombinant bacteriophage M13 plaques should be picked as soon as possible , since the bacteriophage ...
Page 15-70
... plaques usually contain a mixture of both mutant and wild - type sequences . It is therefore essential to plaque - purify the bacteriophages from positively hybridizing plaques as follows : a . Touch the blunt end of a sterile ...
... plaques usually contain a mixture of both mutant and wild - type sequences . It is therefore essential to plaque - purify the bacteriophages from positively hybridizing plaques as follows : a . Touch the blunt end of a sterile ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml