Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-24
... plates . Rinse the plates with ethanol and set them aside to dry . The plates must be free of grease spots to prevent air bubbles from forming in the gel . Treat one surface of each plate with a silicone solution . This prevents the gel ...
... plates . Rinse the plates with ethanol and set them aside to dry . The plates must be free of grease spots to prevent air bubbles from forming in the gel . Treat one surface of each plate with a silicone solution . This prevents the gel ...
Page 12-17
... plate . Do not allow the temperature of the plates to drop below 37 ° C ; otherwise , the growth of the bacteriophages will be retarded . 6. Incubate the plates for at least 4 hours at 37 ° C . If the library is not to be screened in ...
... plate . Do not allow the temperature of the plates to drop below 37 ° C ; otherwise , the growth of the bacteriophages will be retarded . 6. Incubate the plates for at least 4 hours at 37 ° C . If the library is not to be screened in ...
Page 13-56
... plate uppermost . Remove any remaining pieces of electrical tape . Using the end of a metal spatula , slowly pry apart the plates of the mold . With luck , the gel will remain attached to the lower plate . If it does not , place the plate ...
... plate uppermost . Remove any remaining pieces of electrical tape . Using the end of a metal spatula , slowly pry apart the plates of the mold . With luck , the gel will remain attached to the lower plate . If it does not , place the plate ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml