Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-24
... plates , clean the plates with KOH / methanol , which is prepared by adding ~ 5 g of KOH pellets to 100 ml of methanol . Caution : Handle the KOH and the KOH / methanol solutions with great care . Use gloves and a face protector . Then ...
... plates , clean the plates with KOH / methanol , which is prepared by adding ~ 5 g of KOH pellets to 100 ml of methanol . Caution : Handle the KOH and the KOH / methanol solutions with great care . Use gloves and a face protector . Then ...
Page 12-17
... plate . Wetting action then pulls the filter onto the plate . Do not allow the temperature of the plates to drop below 37 ° C ; otherwise , the growth of the bacteriophages will be retarded . 6. Incubate the plates for at least 4 hours ...
... plate . Wetting action then pulls the filter onto the plate . Do not allow the temperature of the plates to drop below 37 ° C ; otherwise , the growth of the bacteriophages will be retarded . 6. Incubate the plates for at least 4 hours ...
Page 13-48
... plates required to accommodate the sequenc- ing reactions . If it is necessary to remove old silicon from the plates , swab them with KOH / methanol , which is prepared by adding ~ 5 g of KOH pellets to 100 ml of methanol . Caution ...
... plates required to accommodate the sequenc- ing reactions . If it is necessary to remove old silicon from the plates , swab them with KOH / methanol , which is prepared by adding ~ 5 g of KOH pellets to 100 ml of methanol . Caution ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml