Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 14-7
... polymerase chain reaction to generate probes for genes or cDNAs that have not yet been cloned . For example , when a limited amount of amino acid sequence is available from the protein of interest ... Polymerase Chain Reaction 14.7.
... polymerase chain reaction to generate probes for genes or cDNAs that have not yet been cloned . For example , when a limited amount of amino acid sequence is available from the protein of interest ... Polymerase Chain Reaction 14.7.
Page 14-16
... reaction , and any change in their concentration affects the concentration of available Mg ++ . We recommend setting up a set of reactions containing fixed concentrations of Tris · Cl ( 10 mm ) and KCl ( 50 mM ) ... Polymerase Chain Reaction.
... reaction , and any change in their concentration affects the concentration of available Mg ++ . We recommend setting up a set of reactions containing fixed concentrations of Tris · Cl ( 10 mm ) and KCl ( 50 mM ) ... Polymerase Chain Reaction.
Page 14-34
... polymerase chain reaction and oligonucleotide probes . Proc . Natl . Acad . Sci . 85 : 1629 . Gibbs , R.A. , P.-N ... polymerase chain reaction and its application to direct sequencing of the HLA - DQA locus . Proc . Natl . Acad . Sci ...
... polymerase chain reaction and oligonucleotide probes . Proc . Natl . Acad . Sci . 85 : 1629 . Gibbs , R.A. , P.-N ... polymerase chain reaction and its application to direct sequencing of the HLA - DQA locus . Proc . Natl . Acad . Sci ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml