Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xviii
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
Page 10-18
... probes , which consist of only one of the two complementary strands of a given sequence of nucleic acid , have a considerable advantage over the more traditional double - stranded probes ... Probes Preparation of Single-stranded Probes 10 18.
... probes , which consist of only one of the two complementary strands of a given sequence of nucleic acid , have a considerable advantage over the more traditional double - stranded probes ... Probes Preparation of Single-stranded Probes 10 18.
Page 10-27
... probes of high specific activity was the development of plasmid vectors containing polycloning sites downstream from powerful promoters derived from the Salmonella ... probes has led Preparation of Radiolabeled DNA and RNA Probes 10.27.
... probes of high specific activity was the development of plasmid vectors containing polycloning sites downstream from powerful promoters derived from the Salmonella ... probes has led Preparation of Radiolabeled DNA and RNA Probes 10.27.
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml