Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 12-2
... proteins in recombinant libraries constructed in expression vectors . In every case , these methods involve screening the library with a ligand that binds specifically to the protein of interest . Such ligands fall into three classes ...
... proteins in recombinant libraries constructed in expression vectors . In every case , these methods involve screening the library with a ligand that binds specifically to the protein of interest . Such ligands fall into three classes ...
Page 15-81
... protein and ( 2 ) X - ray diffraction of protein crystals . Although both of these methods have yielded much information , they can be used only with proteins that are available in large quantity and are of high purity . A less direct ...
... protein and ( 2 ) X - ray diffraction of protein crystals . Although both of these methods have yielded much information , they can be used only with proteins that are available in large quantity and are of high purity . A less direct ...
Page 15-82
... protein to digestion with proteases . Correctly folded proteins are generally more resistant to digestion with proteases such as trypsin and proteinase K than are malfolded proteins . By comparing the ability of mutant and wild - type ...
... protein to digestion with proteases . Correctly folded proteins are generally more resistant to digestion with proteases such as trypsin and proteinase K than are malfolded proteins . By comparing the ability of mutant and wild - type ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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Common terms and phrases
acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml