Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 62
Page 10-2
... radioactive precursors ( usually 32P ) were introduced into cells that were synthesizing the DNA of interest . This procedure required large amounts of radioactivity , involved laborious purifi- cation of the nucleic acids of interest ...
... radioactive precursors ( usually 32P ) were introduced into cells that were synthesizing the DNA of interest . This procedure required large amounts of radioactivity , involved laborious purifi- cation of the nucleic acids of interest ...
Page 11-57
... radioactivity ( by Cerenkov counting , see Appendix E ) in all of the vials . Calculate the proportion of the total radioactivity that has eluted at each temperature ( i.e. , the sum of radioactivity eluted at all temperatures between T ...
... radioactivity ( by Cerenkov counting , see Appendix E ) in all of the vials . Calculate the proportion of the total radioactivity that has eluted at each temperature ( i.e. , the sum of radioactivity eluted at all temperatures between T ...
Page 15-67
... radioactivity at the origin . By measuring the amount of radioactivity at the origin and on the total strip , the percentage of radiolabel transferred from [ y - 32P ] ATP to the oligonucleotide can be calculated . The specific activity ...
... radioactivity at the origin . By measuring the amount of radioactivity at the origin and on the total strip , the percentage of radiolabel transferred from [ y - 32P ] ATP to the oligonucleotide can be calculated . The specific activity ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml