Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 91
Page xviii
... RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 ...
... RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 ...
Page 10-15
... radiolabel . Because the reaction is carried out under conditions that minimize exonucleolytic digestion , the radiolabeled product is not degraded to a detectable extent . If necessary , therefore , the reaction can be allowed to ...
... radiolabel . Because the reaction is carried out under conditions that minimize exonucleolytic digestion , the radiolabeled product is not degraded to a detectable extent . If necessary , therefore , the reaction can be allowed to ...
Page 11-34
... radiolabeled oligonucleotide by centrifugation at 12,000g for 20 minutes at 0 ° C in a microfuge . Using an ... Radiolabeled Oligonucleotides by Precipitation with 34 Purification of Radiolabeled Oligonucleotides by Precipitation with.
... radiolabeled oligonucleotide by centrifugation at 12,000g for 20 minutes at 0 ° C in a microfuge . Using an ... Radiolabeled Oligonucleotides by Precipitation with 34 Purification of Radiolabeled Oligonucleotides by Precipitation with.
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml