Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 81
Page 9-58
... REMOVING PROBES FROM NITROCELLULOSE FILTERS 1. Heat several hundred milliliters of 0.05 × SSC , 0.01 м EDTA ( pH 8.0 ) to boiling . Remove the fluid from the heat and add SDS to a final concen- tration of 0.1 % . Immerse the filter in ...
... REMOVING PROBES FROM NITROCELLULOSE FILTERS 1. Heat several hundred milliliters of 0.05 × SSC , 0.01 м EDTA ( pH 8.0 ) to boiling . Remove the fluid from the heat and add SDS to a final concen- tration of 0.1 % . Immerse the filter in ...
Page 11-36
... remove all of the fluid from the tube , and repeat the washing procedure . 6. Carefully remove all of the fluid from the tube , and add 500 μl of a solution consisting of 80 % ethanol and 20 % 0.1 M sodium acetate ( pH 5.2 ) . Vortex ...
... remove all of the fluid from the tube , and repeat the washing procedure . 6. Carefully remove all of the fluid from the tube , and add 500 μl of a solution consisting of 80 % ethanol and 20 % 0.1 M sodium acetate ( pH 5.2 ) . Vortex ...
Page 12-17
... remove the filters from the solution , and allow them to dry at room temperature on a pad of Kimwipes . Nitrocellulose filters that are free of Triton X - 100 ( Millipore HATF or equivalent ) give the best results . 5. Remove the plates ...
... remove the filters from the solution , and allow them to dry at room temperature on a pad of Kimwipes . Nitrocellulose filters that are free of Triton X - 100 ( Millipore HATF or equivalent ) give the best results . 5. Remove the plates ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml