Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 18
Page v
... Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 Cheenstry Library QH 442.2 526 1989 v . 2 DEVELOPMENT OF PLASMID CLONING VECTORS 1.7 Plasmid Vectors That Permit Histochemical ...
... Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 Cheenstry Library QH 442.2 526 1989 v . 2 DEVELOPMENT OF PLASMID CLONING VECTORS 1.7 Plasmid Vectors That Permit Histochemical ...
Page vii
... Replicating Colonies onto Nitrocellulose Filters 1.93 METHOD 1 1.93 METHOD 2 1.96 Lysis of Colonies and Binding of ... Replication 2.5 Late Transcription 2.5 Assembly 2.7 Lysis 2.8 LYSOGENY 2.8 Bacteriophage A Vectors 2.9 CONSTRUCTION ...
... Replicating Colonies onto Nitrocellulose Filters 1.93 METHOD 1 1.93 METHOD 2 1.96 Lysis of Colonies and Binding of ... Replication 2.5 Late Transcription 2.5 Assembly 2.7 Lysis 2.8 LYSOGENY 2.8 Bacteriophage A Vectors 2.9 CONSTRUCTION ...
Page 10-29
... replication of a single - stranded bac- teriophage . Insertion of foreign DNA sequences into the polycloning site of these vectors can be scored by the appearance of white colonies on plates containing the chromogenic substrate 5 ...
... replication of a single - stranded bac- teriophage . Insertion of foreign DNA sequences into the polycloning site of these vectors can be scored by the appearance of white colonies on plates containing the chromogenic substrate 5 ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml