Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-54
... enzyme of choice for end - labeling DNA molecules with protruding 3 ' tails . 5 ' To Go 5 A DC DT DG DC pA OH 3 ' р ... restriction enzyme and for the subsequent end- labeling . However , not all restriction enzymes work in bacteriophage ...
... enzyme of choice for end - labeling DNA molecules with protruding 3 ' tails . 5 ' To Go 5 A DC DT DG DC pA OH 3 ' р ... restriction enzyme and for the subsequent end- labeling . However , not all restriction enzymes work in bacteriophage ...
Page 15-9
... restriction enzyme and DNA . The eighth tube , which should not receive any restriction enzyme , is used as a control . e . Transfer all eight tubes simultaneously from the ice to a water bath set at the appropriate temperature for the ...
... restriction enzyme and DNA . The eighth tube , which should not receive any restriction enzyme , is used as a control . e . Transfer all eight tubes simultaneously from the ice to a water bath set at the appropriate temperature for the ...
Page 15-88
... restriction site carried by the synthetic hexameric linker and that contains ... enzyme that will generate a fragment of DNA carrying the kan ' gene . Purify ... restriction enzyme that cleaves that target DNA many times and generates ...
... restriction site carried by the synthetic hexameric linker and that contains ... enzyme that will generate a fragment of DNA carrying the kan ' gene . Purify ... restriction enzyme that cleaves that target DNA many times and generates ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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Common terms and phrases
acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml