Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-55
... samples . Excess TBE should be ejected before the next sample is drawn into the syringe and needle . • An automatic micropipettor ( 20 μl ) equipped with a standard tip . Because the tip is too large to fit between the glass plates , the ...
... samples . Excess TBE should be ejected before the next sample is drawn into the syringe and needle . • An automatic micropipettor ( 20 μl ) equipped with a standard tip . Because the tip is too large to fit between the glass plates , the ...
Page 13-94
... samples obtained by the procedures described on pages 13.88-13.93 in 100 μl of 1 м piperidine . 2. Close the tops of the tubes securely . If necessary , centrifuge the tubes briefly to deposit all of the fluid on the bottom . 3 ...
... samples obtained by the procedures described on pages 13.88-13.93 in 100 μl of 1 м piperidine . 2. Close the tops of the tubes securely . If necessary , centrifuge the tubes briefly to deposit all of the fluid on the bottom . 3 ...
Page 15-40
... samples ( 160 μl ) after 15 minutes and 60 minutes of incubation at 37 ° C and place each sample in EDTA , Tris Cl as de- scribed in c . 1x Nuclease - S1 buffer 50 mM sodium acetate ( pH 5.7 ) 200 mM NaCl 1 mм ZnSO4 0.5 % glycerol ...
... samples ( 160 μl ) after 15 minutes and 60 minutes of incubation at 37 ° C and place each sample in EDTA , Tris Cl as de- scribed in c . 1x Nuclease - S1 buffer 50 mM sodium acetate ( pH 5.7 ) 200 mM NaCl 1 mм ZnSO4 0.5 % glycerol ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml