Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 7
Page 9-7
... foreign DNA . This increased capacity allows a modest reduction in the number of recombinant clones that need be constructed and screened to isolate a particular segment of the eukaryotic genome . More significant , however , are the ...
... foreign DNA . This increased capacity allows a modest reduction in the number of recombinant clones that need be constructed and screened to isolate a particular segment of the eukaryotic genome . More significant , however , are the ...
Page 9-8
... segments of foreign DNA . In some cases , the sequence of the stuffer fragment may not be available , but this is usually not relevant . • The presence of amber mutations in the arms . Amber mutations are carried in essential genes in ...
... segments of foreign DNA . In some cases , the sequence of the stuffer fragment may not be available , but this is usually not relevant . • The presence of amber mutations in the arms . Amber mutations are carried in essential genes in ...
Page 15-60
... segment of foreign DNA carried in the vector is sequenced to eliminate the possibility of adventitious mutations at ectopic sites . Restriction enzymes are used to recover the foreign DNA ( or a subfragment carrying the desired mutation ) ...
... segment of foreign DNA carried in the vector is sequenced to eliminate the possibility of adventitious mutations at ectopic sites . Restriction enzymes are used to recover the foreign DNA ( or a subfragment carrying the desired mutation ) ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml