Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 80
Page 11-5
... sequences of these oligonucleotides are typically deduced , using the genetic code , from short tracts of amino acid sequence determined by the sequencing of small quantities of highly purified proteins ( see , e.g. , Hewick et al ...
... sequences of these oligonucleotides are typically deduced , using the genetic code , from short tracts of amino acid sequence determined by the sequencing of small quantities of highly purified proteins ( see , e.g. , Hewick et al ...
Page 13-18
... sequencing , which might generate up to 15 kb of nucleotide sequence , therefore involves • 1 day to prepare single - stranded DNA templates • 1 day of DNA sequencing • 1 day of reading primary DNA sequences and aligning them • 2 days ...
... sequencing , which might generate up to 15 kb of nucleotide sequence , therefore involves • 1 day to prepare single - stranded DNA templates • 1 day of DNA sequencing • 1 day of reading primary DNA sequences and aligning them • 2 days ...
Page 14-30
... sequences of interest in the original sample of nucleic acids . This is because the efficiency of the amplification process is greatly affected by small differences in any one of a number of variables . These include ( 1 ) the quality ...
... sequences of interest in the original sample of nucleic acids . This is because the efficiency of the amplification process is greatly affected by small differences in any one of a number of variables . These include ( 1 ) the quality ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml