Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxiii
... Sequencing Reactions 13.61 DIDEOXY - MEDIATED SEQUENCING REACTIONS USING SEQUENASES 13.65 Preparation 13.65 Sequencing Reactions 13.67 14 13.70 SEQUENCING DENATURED DOUBLE - STRANDED DNA TEMPLATES Sequencing of Plasmid DNAs Purified by ...
... Sequencing Reactions 13.61 DIDEOXY - MEDIATED SEQUENCING REACTIONS USING SEQUENASES 13.65 Preparation 13.65 Sequencing Reactions 13.67 14 13.70 SEQUENCING DENATURED DOUBLE - STRANDED DNA TEMPLATES Sequencing of Plasmid DNAs Purified by ...
Page 13-42
... sequencing reactions . Before setting up sequencing reactions , it is advisable to check the amount of single - stranded template in each preparation of single - stranded bac- teriophage M13 or phagemid DNA . This can be done by ...
... sequencing reactions . Before setting up sequencing reactions , it is advisable to check the amount of single - stranded template in each preparation of single - stranded bac- teriophage M13 or phagemid DNA . This can be done by ...
Page 13-43
... reactions , in which the annealed oligonucleotide is extended and the newly synthesized chains of DNA are terminated by incorporation of ddATP , ddGTP , ddCTP , or ddTTP . In sequencing reactions using Sequenases , a preliminary step ...
... reactions , in which the annealed oligonucleotide is extended and the newly synthesized chains of DNA are terminated by incorporation of ddATP , ddGTP , ddCTP , or ddTTP . In sequencing reactions using Sequenases , a preliminary step ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml