Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-14
... specific activity of the product depends on the amount of template added to the reaction : the lower the amount of template , the greater the specific activity . Under the reaction conditions described , radiolabeling to maximum specific ...
... specific activity of the product depends on the amount of template added to the reaction : the lower the amount of template , the greater the specific activity . Under the reaction conditions described , radiolabeling to maximum specific ...
Page 10-43
... specific for the gastrulation stage of Xenopus development ( Sargent and Dawid 1983 ) , for murine T lymphocytes ( Davis et al . 1984 ; Hedrick et al . 1984 ) , and for rat forebrain ( Rhyner et al . 1986 ) ... SPECIFIC ACTIVITY 10 43.
... specific for the gastrulation stage of Xenopus development ( Sargent and Dawid 1983 ) , for murine T lymphocytes ( Davis et al . 1984 ; Hedrick et al . 1984 ) , and for rat forebrain ( Rhyner et al . 1986 ) ... SPECIFIC ACTIVITY 10 43.
Page 11-41
... specific activity of the [ a - 32P ] dNTPs in the reaction . The specific activity of a dNTP whose a - phosphate has been completely substituted by 32P is approximately 9000 Ci / mmole . Preparations with lower specific activities ...
... specific activity of the [ a - 32P ] dNTPs in the reaction . The specific activity of a dNTP whose a - phosphate has been completely substituted by 32P is approximately 9000 Ci / mmole . Preparations with lower specific activities ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml