Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-56
... stock solution at 440 nm . The molar extinction coefficient of pure ac- tinomycin D ( m.w. = 1255 ) in aqueous solution is 21,900 . The absorbance at 440 nm of a solution containing 1 mg / ml of the drug is therefore 0.182 . Stock solutions ...
... stock solution at 440 nm . The molar extinction coefficient of pure ac- tinomycin D ( m.w. = 1255 ) in aqueous solution is 21,900 . The absorbance at 440 nm of a solution containing 1 mg / ml of the drug is therefore 0.182 . Stock solutions ...
Page 10-7
... solution into small aliquots and store them at −20 ° C . 10 × Ligation buffer 0.5 M Tris Cl ( pH 7.6 ) 100 mM MgCl , 100 mм dithiothreitol 500 μg / ml bovine serum albumin ( Fraction V ... Stock Solutions Used in Nick Translation 10 7.
... solution into small aliquots and store them at −20 ° C . 10 × Ligation buffer 0.5 M Tris Cl ( pH 7.6 ) 100 mM MgCl , 100 mм dithiothreitol 500 μg / ml bovine serum albumin ( Fraction V ... Stock Solutions Used in Nick Translation 10 7.
Page 13-85
... stock solution Lyophilizer Microfuge tubes Sequencing gels and electrophoresis apparatus Temperature control Dowex XG8 mixed - bed resin for 1 hour and filtering twice through Whatman No. 1 paper . Deionized formamide should be stored ...
... stock solution Lyophilizer Microfuge tubes Sequencing gels and electrophoresis apparatus Temperature control Dowex XG8 mixed - bed resin for 1 hour and filtering twice through Whatman No. 1 paper . Deionized formamide should be stored ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml