Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 63
Page 9-49
... stored at -20 ° C . The stock solution is diluted tenfold into prehybridization buffer ( usually 6 × SSC or 6 × SSPE containing 0.5 % SDS and 100 μg / ml denatured , fragmented , salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g ...
... stored at -20 ° C . The stock solution is diluted tenfold into prehybridization buffer ( usually 6 × SSC or 6 × SSPE containing 0.5 % SDS and 100 μg / ml denatured , fragmented , salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g ...
Page 13-48
... stored at 4 ° C for several weeks . 6 % Acrylamide / urea top solution 40 % acrylamide solution 5x TBE urea ( ultrapure ) 75 ml 50 ml 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a ...
... stored at 4 ° C for several weeks . 6 % Acrylamide / urea top solution 40 % acrylamide solution 5x TBE urea ( ultrapure ) 75 ml 50 ml 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a ...
Page 13-85
... stored in small aliquots under nitrogen at -70 ° C . Several companies sell formamide , distilled and packaged under nitrogen , that need not be purified before use ( e.g. , BRL catalog no . 5515UA ) . At least 100,000 dpm ( 25,000 ...
... stored in small aliquots under nitrogen at -70 ° C . Several companies sell formamide , distilled and packaged under nitrogen , that need not be purified before use ( e.g. , BRL catalog no . 5515UA ) . At least 100,000 dpm ( 25,000 ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
82 other sections not shown
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml